BFSRI/Tinami Cancer Clinic

Monocytes are collected from patients and dendrocytets (DC) cells and other cells including NKT cells are separated. DC is pulsed with cancer antigens for education and cultured with other cells for infusion to the patients. Chinami et.al:Case Report: Assessment by miRNA microarray of an Autologous Cancer Antigen-pulsed Adoptive Immune Ensemble Cell Therapy (AC-ACT) approach; demonstrated induction of anti-oncogenic and anti-PD-L1 miRNAs.

Antioncogenes and anti-sense against oncogenes are introduced by plasmid vectors. They are not integrated into host chromosome and not sel-replicate, thus  give no cancer risks.

clnical specimen: Monocytes collected from 20ml of blood are sent and cultured.

The procedures for preparing the samples were performed within a high efficiency particle arrestance (HEPA)-filter clean-air barriered good manufacturing practice (GMP) cell processing facility.  A standard operation procedure (SOP) for the production was established according to institutional GMP-based guidelines.

Staffs

CEO: Masanobu Chinami, MD&PhD ; aciam@crux@ocn.ne.jp

Research Director: Chaker N. Adra,PhD(left picture); cnadra@crux@ocn.ne.jp; (Ref:https://www.ncbi.nlm.nih.gov/pubmed/?term=Adra+CN)

Research Director:Kaoru Iwabuchi, PhD

Reserch associate:Miki Nakashima

Excellent works done by Chaker Adra

(1) Pregnenolone sulfate “The Brain Steroid”, one of steroid metabolites was found to be effective for Alzheimer and other neurodegenerative disease (1). Steroids have many effects such as anti-inflammatory, immune-suppressive effects, and so far. This formula of compound specifically enter into nAdra CN, Zhu S, Ko JL, Guillemot JC, Cuervo AM, Kobayashi H, Horiuchi T, Lelias JM, Rowley JD, Lim B. LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. Genomics. 1996;35:328–337. euronal cell and give effects to the cells.
(2) αB-crystallin, one of heat shock proteins, when used at severe burn, was found to have remarkable effects of suppression SIRS (systemic inflammatory response syndrome) and rescue lives of mice (2).  Spraying of αB-crystalline proteins in cases of severe burn has a possibility of great improvement for rescue rate in human and other farm animals.
(3) SMARCAD1, a human helicase gene was cloned (3 al.Genomics. 2000 ) and this is highlighted as “finger print gene” and also  (4,5 Nature. 2012 )
(4) HTm4 gene was cloned (6 Proc Natl Acad Sci U S A. 1994 ). This is similar to IgEFcεRIβ which is associated with allergy and atopic diseases and also cycle regulation (7J Biol Chem. 2005)
(5) Promotor gene of PGK1(phosphoglycerate kinase1 ) was cloned (8 Gene 1987) and this is widely used for gene therapy as (pgk1)-Neo Cassette/Shuttl (9 J. Virol. 1996)
(6) PGK2 gene was found to exist specifically in the testis autosomal chromosome (10 . Mol Cell Biol. 1987). This gene has no introns different from PGK1, which demonstrates PGK2 evolved in the very old time by retrotransposon afterbarising from PGK1.

(7)  LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. Genomics. 1996;35:328–337. Adra et.al

clnical specimen: Monocytes collected from 20ml of blood are sent and cultured.

The procedures for preparing the samples were performed within a high efficiency particle arrestance (HEPA)-filter clean-air barriered good manufacturing practice (GMP) cell processing facility.  A standard operation procedure (SOP) for the production was established according to institutional GMP-based guidelines.

Staffs

CEO: Masanobu Chinami, MD&PhD ; aciam@crux@ocn.ne.jp

Research Director: Chaker N. Adra,PhD(left picture); cnadra@crux@ocn.ne.jp; (Ref:https://www.ncbi.nlm.nih.gov/pubmed/?term=Adra+CN)

Research Director:Kaoru Iwabuchi, PhD

Reserch associate:Miki Nakashima

Excellent works done by Chaker Adra

(1) Pregnenolone sulfate “The Brain Steroid”, one of steroid metabolites was found to be effective for Alzheimer and other neurodegenerative disease (1). Steroids have many effects such as anti-inflammatory, immune-suppressive effects, and so far. This formula of compound specifically enter into nAdra CN, Zhu S, Ko JL, Guillemot JC, Cuervo AM, Kobayashi H, Horiuchi T, Lelias JM, Rowley JD, Lim B. LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. Genomics. 1996;35:328–337. euronal cell and give effects to the cells.
(2) αB-crystallin, one of heat shock proteins, when used at severe burn, was found to have remarkable effects of suppression SIRS (systemic inflammatory response syndrome) and rescue lives of mice (2).  Spraying of αB-crystalline proteins in cases of severe burn has a possibility of great improvement for rescue rate in human and other farm animals.
(3) SMARCAD1, a human helicase gene was cloned (3 al.Genomics. 2000 ) and this is highlighted as “finger print gene” and also  (4,5 Nature. 2012 )
(4) HTm4 gene was cloned (6 Proc Natl Acad Sci U S A. 1994 ). This is similar to IgEFcεRIβ which is associated with allergy and atopic diseases and also cycle regulation (7J Biol Chem. 2005)
(5) Promotor gene of PGK1(phosphoglycerate kinase1 ) was cloned (8 Gene 1987) and this is widely used for gene therapy as (pgk1)-Neo Cassette/Shuttl (9 J. Virol. 1996)
(6) PGK2 gene was found to exist specifically in the testis autosomal chromosome (10 . Mol Cell Biol. 1987). This gene has no introns different from PGK1, which demonstrates PGK2 evolved in the very old time by retrotransposon afterbarising from PGK1.

(7)  LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. Genomics. 1996;35:328–337. Adra et.al